Monday, September 19, 2016

Analysis of CRP = C - Reactive Protein

Published by Unknown on Monday, September 19, 2016  | No comments

Has been discovered in the world of 1930, a protein Penta installation and talents (120KD) this protein found in human blood a few percent, which belongs to Alphglobulin category consists of the liver cells, and specifically in Hepatocytes cells, which does not refer to any specific satisfactory indication of any non-specific and is similar to the analysis ESR but the best of it because it is not affected by anemia or fibrinogen characterized this protein that has recipes Alglupulinat immune and thus has the ability to stacking and sedimentation.
And the normal rate for this protein in the blood: Less than 0.6 dl / mg blood.
Detection of this protein in large quantities and indicates that the patient is suffering from acute inflammation, and this helps the doctor to diagnose many medical conditions that cause high proportion of this protein in the blood:
1. Acute inflammation in the body.
2. rheumatoid arthritis RA.
3. rheumatic fever.
4. brucellosis.
5. malignancies.
6. Wolf erythema systemic SLE.
7. acute hepatitis.
8. myocardial infarction.
9. cell death.
10. gout.
And other pathological conditions.
- Work CRP test method:
1. attend all of the solutions, and the patient's serum (Serum) at room temperature.
2. placed 40 microliter of Positive Control in the first room on the latex segment and 40 microliter of Negative Control in the second chamber in order to work out after comparing the results to ensure the validity of the solutions.
3. placed 40 microliter of Serum patient in one room.
4. Adavo 40 microliter of latex solution (one drop) on the Positive Control and the Negative Control Serum and the patient.
5. blends in a circular motion using the appropriate sticks.
6. placed on the slide latex rocking Shaker for 1-3 minutes stacking appears under the light is not taken as a result after this time because it starts in the dry samples which may lead to a false positive agglutination Positive False.
• (+): the occurrence agglutination Agglutination (Ab-Ag Reaction).
• (-): agglutination Non Agglutination does not happen (Ab-Ag Non Reaction)

A picture of the details of CRP in the case of stacking and in the absence of stacking.
In the event of agglutination Agglutination is done easing to give a numeric result

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