The goal of the test:
This test determines the presence of sickle cell anemia or lack of it, and sickle cell anemia cases of anemia, in which red blood cells appear in the form of a sickle.
And that because of the amount of Alimo Zhlobin abnormal that lead to deposition on the crystal form in the case of lack of oxygen in the blood which gives red blood cells sickle shape
There are two ways to detect sickle cell laboratory:
The first way to use Sickling solution lotion - (Solubility Method) 1:
* The method of work:
1. placed 2 mm of the solution Sickling solution in a test tube clean.
2. placed 25 microliter blood sample slowly to the previous solution.
3. ETT is moving very gently until combined amount of blood in solution.
4. leave the tube for 5 minutes at room temperature 'and then read the result after that.
* How to read the result:
- When there is turbidity in the tube so that if two lines are put in black as a backdrop to the tube can not be seen clearly considered a positive result.
- If the net or appealing tube and can see the lines are considered negative result.
* Explain the impact of the solution:
Sickling solution the solution works to break down red blood cells and thus in the presence of hemoglobin (S) of the solution becomes turbid because this type of hemoglobin in solution for Aivob (Sickling solution).
In the case of a normal hemoglobin, it dissolves in the solution and become Tube net.
The second way to use the glass slide (slide):
* The method of work:
1. a drop of blood placed in the middle of a clean glass slide.
2. placed the slide cover (Cover glass) in a manner to prevent air bubbles are under the hood.
(In the case of air bubbles can be pressure on the lid until the bubbles out).
3 is placed (Vaseline or wax or Alkenda balm) on the edges of the (Cover glass) until air Aatserb to the inside.
4. leave the glass slide in incubation at 37 ° C for 24 hours.
5. After incubation examine the slide under the microscope lens oily.
* How to read the result:
- In the case of positive red blood cells take the form of a sickle.
- In the negative case does not change the form of red blood cells and remain as they are.
* Explain what is happening in this way:
Prevent the (Cover glass) with a wax on the outer edges him from entering the oxygen to the inside as the increase in the period of incubation for 24 hours lead to oxygen under the slide the cover consumption (Cover glass) and thus in the case of red blood cells sickle it deposition occurs hemoglobin inside them as a result of lack of oxygen made in the form of giving them a machete.
In the case of a natural red blood cells, it can not happen when the change in the form of lack of oxygen.
It is added to the solution (Sodium dithionite) to the blood drop this solution helps to pull oxygen from the blood and thus lead to take the red sickle shape in a short period of blood cells, and in this case can read the test result within an hour of time.
0 التعليقات: