Use of anticoagulants in the case of the use of samples of plasma or total blood as required by the experiment and it must add an anti-coagulant to the tube blood collection case withdrawn immediately and usually closes the wall of a tube of blood collection in anti clotting, and it should be noted that the selection of anticoagulation should be based on the grounds this counter will not affect the chemical analysis and this point is very important. Because the sources of coagulation are chemical compounds of salts of some metals such as sodium, potassium, lithium, so it can not be used anticoagulants sodium and potassium salts when the terms of the analysis set electrolytes Kalsodiom and potassium because it will lead to a greater positive error in the results of the analysis but in such a case can be used anticoagulants for lithium or ammonium.
In the case of the analysis of calcium in the blood can be used sodium oxalate salt because this will remove all its contents Petrsiba sample of calcium in the form of calcium oxalate is not.
As well as anticoagulants to discourage the effectiveness of some enzymes, such as the enzyme phosphatase Acid Acid Phosphatase and phosphatase baseband Alkaline Phosphatase and enzyme dehydrogenase of lactate LDH The salts of potassium fluoride or sodium Vtthbt effectiveness of the enzyme urease, while operating effectiveness of the enzyme amylase, can also anticoagulants discredit the test pathological significance.
These are some of the types of materials coagulase blood:
1 - heparin Heparin:
Is antimatter-clotting, one of the basic components of blood, but there is a concentration is not enough to prevent blood clotting, Witold heparin from the liver cells it is high concentration in the liver and is also present in the pulmonary cells has been possible to disconnect and isolate urgently amorphous liver extract, Lung, and is distinguished from the other being that does not interfere with any test of chemical analysis tests, heparin is a Mecotiin many sulfuric acid Muccoitin Polysulphouric - acid, a multi-sugars and can be obtained commercially at present of sodium salts sodium heparin or potassium salt potassium heparin or lithium salt lithium heparin
Heparin acts as an thrombin Antithrombin where prohibited transfer or convert prothrombin to thrombin Prothrombin Thrombin and so prevents the formation of fibrin Fibrin and Fibrinogen fibrinogen to be clotting process in two stages as follows:
Prothrombin Thrombplastic Activity Factor ► Thrombin
Fibrinogen Thrombin ► Fibrin (blood colt)
And heparin needs to Assistant Cofactor worker to do his job.
Heparin is added 20% units per ml of blood, including that it does not dissolve in the case, therefore, of solution is often used and dried, Alr the walls of the tube to be in direct contact with the blood and the effect the best you can, and still high oil prices and temporary effect of the obstacles used in laboratories .bamadadat when compared to other anticoagulants, it contains sodium heparin at least 110 units / mg is usually used concentration of about 0.2 mg / ml of blood.
2 - potassium oxalate Potassium Oxalates:
This works a counter on the deposition of ions of calcium and thus prevents blood clots and preferably used for easy melting, usually we need 10-20 mg of oxalate a potassium to prevent clot 10 mL of blood and 2 mg per one ml of blood is used this is usually a concentration of 30% converter and titrate to a pH PH = 7.4 by adding potassium hydroxide solution or oxalic acid solution is worth mentioning that 0.1 ml of potassium oxalate solution reddish enough to prevent clotting 10 ml of blood.
3 - Sodium fluoride Sodium Fluoride:
Commonly used as a preservative for glucose estimation in the blood, but it is used as an anti-clotting (weak), and when used as a preservative in addition to the presence of anticoagulant such as potassium oxalate, it would be an effective concentration of about 2 mg / 1 ml of blood and starts its effect by inhibiting system co-enzyme in the Glycolysis process which leads to a lack of focus, and attend pipe container for this to the mix by dissolving 4 g of sodium chloride with 12 g of potassium oxalate in 200 ml of water, one drop placed in each tube per 1 mL of blood and dried tubes to a lesser extent from the 100-meter heat.
As a general rule Viz fluoride it should not be used when collecting samples for enzymatic estimates or when used in the test reagent Reagent (length enzyme), such as a way to estimate the urease Urease urea.
4 - ethylene diamine four-acetic acid (Ethylene Diamine Tetra Acetic Acid (EDTA:
Preferably use this counter in blood tests aware of Hematology, in particular, where he works to maintain the cellular components from damage and is generally used in a disodium salt or dual-potassium concentration of approximately 1-2 mg / ml of blood and attributed the effectiveness of this Aalmlh as an anti-coagulant to its ability to link with blood calcium and isolated completely from playing its role in the coagulation process.
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